Digital One Health Nanopore Workshop
Date: 26-27 February 2024
Location: National Health Laboratory and Diagnostic Services (NHLDS), Kampala, Uganda
Overview
This workshop will provide hands-on experience preparing bacterial isolates for whole genome sequencing on the Oxford Nanopore sequencing platform. Colleagues across the One Health ministires will be able to use this to improve the resolution of AMR surveillance. This is part of a series of initiatives from the Digital One Health Laboratory that aims to bring together AMR stakeholders from various institutions across the One Health spectrum to increase the AMR surveillance capacity in Uganda and the region.
Photo gallery
NHLDS, Kampala
NHLDS, Kampala
NHLDS, Kampala
NHLDS, Kampala
NHLDS, Kampala
NHLDS, Kampala
NHLDS, Kampala
NHLDS, Kampala
NHLDS, Kampala
NHLDS, Kampala
Links (For Workshop Use)
Google Drive - Workshop material
Collaborative Google Doc ← link not active yet
Programme
Day 1
| Time | Session | Who |
| 09:00 | Registration and morning coffee | All |
| 09:15 | Opening ceremony | All, Dr Susan Nabadda, Dr Adrian Muwonge |
| 09:45 | Part 1: DNA Extraction 1 (3 Hours) | 11 participants and trainers only |
| 12:45 | Lunch Break | 11 participants and trainers only |
| 13:30 | Part 2: DNA Extraction 2 (2 Hours) | 11 participants and trainers only |
| 15:30 | Afternoon break | 11 participants and trainers only |
| 16:00 | Digital One Health showcase (Bodastage) | 11 participants and trainers & Emmanuel Ssebaggala |
| 17:00 | END | Everyone |
Day 2
| Time | Session | Who |
| 09:00 | Arrival and morning coffee | 11 participants and trainers only |
| 09:30 | Bioinformatics overview (MinKnow overview) | 11 participants and trainers only |
| 10:30 | Part 3: Preparing a sequencing library (2 hours) | 11 participants and trainers only |
| 12:30 | Lunch | 11 participants and trainers only |
| 13:30 | Part 4: Starting a sequencing Run (1.5 hours) | 11 participants and trainers only |
| 15:00 | Afternoon tea | 11 participants and trainers only |
| 15:30 | Bioinformatics overview (EPI2ME) | 11 participants and trainers only |
| 16:30 | END | Everyone |
Trainers
- Adrian Muwonge : The Roslin Institute, University of Edinburgh, UK
- Bryan Wee: The Roslin Institute, University of Edinburgh, UK
- Vesa Qarkaxhija: The Roslin Institute, University of Edinburgh, UK
- Frank Chilanga: The Roslin Institute, University of Edinburgh, UK
- Emmanuel Ssebaggala : Bodastage Solutions, Uganda
Workshop participants
Animal health
- Arinaitwe Eugene : National Animal Disease Diagnostic and Epidemiology Center (NADDEC), Uganda.
- Gladys Nakanjako Kiggundu : National Animal Disease Diagnostic and Epidemiology Center (NADDEC), Uganda.
- Esther Nabattta : National Animal Disease Diagnostic and Epidemiology Center (NADDEC), Uganda.
Environmental health
- George Olum : Ministry of Water and Environment (MWE), Uganda.
- Ankunda Penrose : Ministry of Water and Environment (MWE), Uganda.
- Godfrey Katumba : Ministry of Water and Environment (MWE), Uganda
Human health
- Daniel Eurien : Central Public Health Laboratories (CPHL), Uganda.
- Lydia Bulyaba : Central Public Health Laboratories (CPHL), Uganda.
- Julius Sseruyange : Central Public Health Laboratories (CPHL), Uganda.
- Godwin Tusabe : Central Public Health Laboratories (CPHL), Uganda.
One Health:
- Kia Praiscillia : Makerere University, Uganda
- Franklin Mayanja : Fleming Fellowship Alumni, National Animal Disease Diagnostic and Epidemiology Center (NADDEC), Uganda.
Registration
For any inquiries, please contact Adrian Muwonge.
Partners
We’d like to thank the Royal Society and the Fleming Fund for making this event possible.
